Title: Development and characterization of EST-SSR markers in the invasive weed Mikania micrantha (Asteraceae)
Abstract: Microsatellite markers were developed for the invasive species Mikania micrantha to investigate its invasion history. Transcriptome sequencing projects offer apotential source for the development of new markers. Sixteen polymorphic simple sequencerepeat (SSR) markers derived from ESTs were identified and screened in 36 samples of M. micrantha. The number of alleles per locus ranged from two to four, with an average of 2.7 alleles perlocus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.7754 and 0 to 0.8333, respectively. These EST-SSR markers may be useful for further investigation of the population genetics of M. micranthaas additional tools to trace its invasion history. [Yubin Yan, , Xiaoting Fang, Lu Lu, , Xuejun Ge & Suhua Shi (2011).Development and characterization of EST-SSR markers in the invasive weed Mikania micrantha (Asteraceae). American Journal of Botany, on-line 24 Nov. 2010, doi:10.3732/ajb.1000387.
Keywords: expressed sequence tag • invasive species • microsatellite • Mikania micrantha