Abstract: We tested the presence of glyphosate in the urines of a farmer who sprayed a glyphosate based herbicide on his land, and in his family, as his children were born with birth defects that could be due to or promoted by pesticides. Glyphosate residues were measured in urines a day before, during, and two days after spraying, by liquid chromatogra-phy-linear ion trap mass spectrometry. Glyphosate reached a peak of 9.5 μg/L in the farmer after spraying, and 2 μg/L were found in him and in one of his children living at a distance from the field, two days after the pulverization. Oral or dermal absorptions could explain the differential pesticide excretions, even in family members at a distance from the fields. A more detailed following of agricultural practices and family exposures should be advocated together with in-formation and recommendations. [R. Mesnage, C. Moesch, R. Grand, G. Lauthier, J. Vendômois, S. Gress & G. Séralini (2012). Glyphosate Exposure in a Farmer’s Family, Journal of Environmental Protection, 3(9), 1001-1003. doi: 10.4236/jep.2012.39115.] Comment